Download the Cellastim S Reconstitution Application Note

Reconstitution of Cellastim S (recombinant human serum albumin) for cell-culture applications  

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Blood-Free LogoInVitria's Cellastim S Reconstitution Application Note Includes:

  • Workflow Summary
  • Materials Needed
  • Protocol

Introduction

Human serum albumin (HSA) has been identified as a powerful tool for the expansion of many cell types in vitro. HSA is a multifaceted protein capable of binding many different types of molecules. HSA therefore plays diverse roles in cell biology, acting as an antioxidant to improve health of cells by binding to reactive oxygen species, as an energy-delivery system by chaperoning fatty acids in the bloodstream, and as a mediator for solubilization and stabilization of metal ions required for cellular processes. These functions make HSA supplementation of cell culture media an integral step in facilitating cell growth and expansion in vitro, especially when removing serum or blood-derived proteins for use in clinical manufacturing.

Cellastim S is a completely blood-free and animal-free human albumin produced in a scalable, non-mammalian recombinant expression system. It is naturally free of prion agents (bovine spongiform encephalopathy and other transmissible spongiform encephalopathy agents), and carries less potential risk from other mammalian adventitious agents compared to serum-derived components.

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